Beschreibung
The creatinine urine adulteration test can be used to check whether a urine sample has been falsified for drug analysis . On the basis of an easy-to-perform urine sample and the comparison using a color scale, a possible urine manipulation can be determined.
In adulteration, a urine sample is deliberately manipulated to falsify and alter test results. The use of adulterants is often used to induce false-negative results in drug tests. This is done by manipulating the test and/or destroying the substances present in the urine. Dilution of the urine can also lead to false-negative test results.
One of the best methods to detect adulteration or dilution is to determine certain urine properties,
such as creatinine, pH and specific gravity, and to detect the presence of nitrite, glutaraldehyde and
oxidants/pyridinium chlorochromate in the urine.
Test indicators urine manipulation test
Creatinine (CREA): Creatinine is a waste product of creatine and an amino acid found in muscle tissue and present in urine. The consumption of very high amounts of liquids such as e.g. Water or teas can lead to the flushing out of the drug waste products. Creatinine and specific density are therefore two methods to test for dilution and flushing, which are the two most common mechanisms for manipulating drug tests. Low creatinine levels or low density can be an indication of diluted urine.
Nitrite (NIT): The test for nitrite can be used to determine whether additional adulterants have been added to the urine. Urine should not contain any traces of nitrite. Positive results generally indicate the presence of an added adulterant.
Glutaraldehyde (GLUT): Adulterants may contain glutaraldehyde, which will destroy the enzyme used in some immunoassays, which can lead to false drug results. Glutaraldehyde is also not a component of natural urine. The detection of glutaraldehyde in a urine sample therefore generally indicates adulteration.
pH value: The pH value is used to determine the detection of acidic or alkaline adulterants in the urine. Normal pH values should be in the range of 4.0 – 9.0. Values outside this range may indicate that the sample has been tampered with.
Specific Gravity (SG): Based on the specific density, the urine is tested for sample dilution. Deviating values from the norm indicate a dilution of the urine.
Oxidants/pyridinium chlorochromate (OXI/PCC): Oxidizing reagents such as bleach or
hydrogen peroxide are tested. Pyridinium chlorochromate is a frequently used counterfeiting agent. Natural human urine does not contain oxidants
or PCC.
